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        Français

        PSB

        Carl-Ivar Br?ndén building (CIBB)
        71, Avenue des Martyrs
        CS 90181
        F-38042 Grenoble
        Intranet

        RoBioMol

        Purpose of the platform (operated by IBS)

        Molecular Biology and Protein Expression Automated Platform

        RoBioMol does the following steps using robots and dedicated software:

        - Design two oligos for PCR amplification of selected gene
        - Clone gene into a choice of expression vectors
        - Sequence two positive clones
        - Fractionate expression strain
        - Small‐scale affinity purification (insoluble and soluble fractions)
        - Analyses purified protein fractions using SDS‐PAGE and Western blot
        - Provides a detailed report (with sequence validation of the gene)
        - Delivers the expression system (plasmid)


        Web page of the platform

        webpage


        Responsible of the platform

        Thierry Vernet


        Staff in charge of the platform

        Anne-Marie Villard


        In practice

        Access mode: Service and collaborations

        How to make a request: e-mail to robiomol@ibs.fr to
        Collaborations:
        Upon selection by a scientific committee. For selection criteria and application procedure please contact vernet@ibs.fr
        Acknowledgements in publication:
        “This work used the platforms of the Grenoble Instruct centre (ISBG ; UMS3518 CNRS-CEA-UGA-EMBL) with support from FRISBI (ANR-10-INBS-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB).
        We thank Anne Marie Villard and Dr. Marjolaine Noirclerc-Savoye, for the “gene cloning, site-directed mutagenesis, protein purification or Thermal Shift Assay experiments ; and Céline Charavay and Stéphane Segard (GIPSE, CEA, Grenoble) for the RoBioLIMS database development”.


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