<form id="1557r"></form>

    <sub id="1557r"></sub>

    <thead id="1557r"></thead>
    <sub id="1557r"></sub>

      <thead id="1557r"></thead>

        Français

        PSB

        Carl-Ivar Br?ndén building (CIBB)
        71, Avenue des Martyrs
        CS 90181
        F-38042 Grenoble
        Intranet

        High Throughput Membrane Protein Crystallization Platform

        Purpose of the platform (operated by IBS)


        The High Throughput Membrane Protein Crystallization (HTMPC) platform is fully equipped to accomplish successful crystallization of your membrane protein (in meso, bicelle, vapor diffusion). Robotic systems nanovolume dispenser (NT8 from Formulatrix) allows to screen 96 precipitant conditions with small amount of sample (5-10 μl per plate) in less than 10 minutes. Our platform works with commercially available kits specialized in membrane protein crystallization. An automated imaging system, RockImager 1500 (Formulatrix), visualizes crystallization drops with visible, polarized and UV lights, which allows a better identification of protein crystals. The smallest crystals which can be confidently detected are about 10 micrometers.

        The diffusion rate and mobile fraction of the membrane protein can be determined by FRAP (Formulatrix), that allows to identify the optimal conditions for LCP crystal growth. In addition, FTIR (Brucker) and UV-Vis specrophotometer (Schimadzu) are available at the platform for precise determination of detergent and membrane protein content correspondingly.


        Responsible

        Florine Dupeux


        Staff in charge of the platform

        Florine Dupeux


        In practice

        - Access mode
        Open access to internal and external users.
        https://www-crims-htmpc.ibs.fr (login: crimsUser; pwd: 6horowitz)

        - Cost
        https://www-crims-htmpc.ibs.fr/index.php?option=com_content&view=article&id=49&Itemid=395

        - Location
        IBS/Room 313
        IBS/Office 357

        - How to make a request?
        Register and send request forms on our website: https://www-crims-htmpc.ibs.fr
        (login: crimsUser; pwd: 6horowitz)

        - Credits
        In the general case users must aknowledge the platform in their publications: “This work used the platforms of the Grenoble Instruct-ERIC Center (ISBG : UMS 3518 CNRS-CEA-UGA-EMBL) with support from FRISBI (ANR-10-INBS-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB)."


        大芭蕉天天视频在线观看